O026 - Unsupervised Stain Decomposition via Inversion Regulation for Multiplex Immunohistochemistry Images

Shahira Abousamra, Danielle Fassler, Jiachen Yao, Rajarsi R. Gupta, Tahsin Kurc, Luisa Escobar-Hoyos, Dimitris Samaras, Kenneth Shroyer, Joel Saltz, Chao Chen

Show abstract - PDF - Reviews

Multiplex Immunohistochemistry (mIHC) is a cost-effective and accessible method for in situ labeling of multiple protein biomarkers in a tissue sample. By assigning a different stain to each biomarker, it allows the visualization of different types of cells within the tumor vicinity for downstream analysis. However, to detect different types of stains in a given mIHC image is a challenging problem, especially when the number of stains is high. Previous deep-learning-based methods mostly assume full supervision; yet the annotation can be costly. In this paper, we propose a novel unsupervised stain decomposition method to detect different stains simultaneously. Our method does not require any supervision, except for color samples of different stains. A main technical challenge is that the problem is underdetermined and can have multiple solutions. To conquer this issue, we propose a novel inversion regulation technique, which eliminates most undesirable solutions. On a 7-plexed IHC image dataset, the proposed method achieves high quality stain decomposition results without human annotation.
Hide abstract

Oral presentation

Schedule: Monday, July 10: Oral session 2 - Unsupervised/weakly supervised methods — 14:00–15:00
Monday, July 10: Posters — 11:00–12:00 & 15:00–16:00
Poster location: M02